Polyvinyl alcohol (PVA) is a water-soluble synthetic resin widely used in coatings. PVA is a potent wastewater pollutant. Acinetobacter, Flavobacterium, and Pseudomonas bacterial strains with alcohol peroxidase activity perform enzymatic mineralization of polyvinyl alcohol (Chandra Rustgi, 1998). Bacillus megaterium, Alcaligenes faecalis, Alcaligenes, Bacillus, Sphingobacterium, Pseudomonas, PVA3, Pseudomonas vesicularis PD, and Pseudomonas O-3 were identified as polyvinyl alcohol degraders (Pajak et al., 2010; Rong et al., 2009; Raghul et al., 2013). Wait. (2009) isolated Novosphingobium sp. P7 and Novosphingobium sp. P7 from activated sludge, showing PVA degradation in the presence of methionine in the medium. Novosphingobium sp. can perform PVA degradation in the absence of methionine. P7 when co-cultured with Flavobacterium B2. Marusincova et al. (2013) isolated Steroidobacterium. Degradation of PVA by PD and Steroidobacterium from a municipal wastewater treatment plant. PD was achieved under both aerobic and denitrifying conditions. PVA degradation by Sphingopyxis sp. The 113P3 strain was reported by Hu et al. (2007). Periplasmic PVA dehydrogenase degrades PVA of lower molecular size more rapidly than PVA of average molecular size.